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trizol method  (TaKaRa)


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    Structured Review

    TaKaRa trizol method
    Trizol Method, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 31112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trizol+method/RNAiso+Plus/pmc13094214-109-9-13
    Average 99 stars, based on 31112 article reviews
    trizol method - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Tributyrin enhances growth and intestinal health in green mud crab ( Scylla paramamosain ) through butyrate-driven metabolic regulation
    Article Snippet: .. Briefly, total RNA (approximately 0.1 g sample) was isolated from the intestine and hepatopancreas of crabs (pooled from two crabs per replicate, n = 6 per treatment) using the Trizol method (RNAiso Plus 9108, Takara Biomedical Technology Co., Ltd., Dalian, Liaoning, China) and quantified via a Spectrophotometer (NanoDrop2000, Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription was carried out to synthesis cDNA following the instructions of the HiScript II Q RT SuperMix (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China).

    Article Title: Indole-3-propionic acid enhances glycolytic myofiber formation in piglets through PI3K-mTOR activation and gut microbiota-driven tryptophan metabolic alteration
    Article Snippet: Plasma glucose concentration was measured using a blood glucose assay kit (Cat. No. ml076792, Shanghai Enzyme-Linked Biological Technology Co., Ltd., Shanghai, China). .. RNA was isolated from the longissimus dorsi using the TRIzol method (Cat. No. 9109, Takara Biotechnology [Dalian] Co., Ltd., Dalian, China). .. It was then converted to cDNA with Hifair III 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus) (Cat. No. 11141ES60, Shanghai Yeasen Biotechnology Co., Ltd., China) and stored at −80 °C.

    Article Title: Novel small molecule derivatives improve survivability in the cellular model of Huntington's disease via improving mitochondrial fusion
    Article Snippet: .. Total RNA was isolated from control and HD cells using the Trizol method (RNAiso Plus-Takara), while the quantity and quality of RNA were determined using NanoDrop (Thermo Scientific). cDNA was prepared using iScriptTM cDNA Synthesis (Bio-Rad) . .. RT-qPCR was carried out in a CFX96-real-time PCR machine (Bio-Rad) using SYBR green master mix (TB Green, Takara) with specific primers for DNM1L (DRP1), MFN1, MFF, FIS1, and PGC1α-Exon1a 25 (primer details in Table S3 under SI).

    Article Title: Tributyrin enhances growth and intestinal health in green mud crab ( Scylla paramamosain ) through butyrate-driven metabolic regulation.
    Article Snippet: .. Briefly, total RNA (approximately 0.1 g sample) was isolated from the intestine and hepatopancreas of crabs (pooled from two crabs per replicate, n = 6 per treatment) using the Trizol method (RNAiso Plus 9108, Takara Biomedical Technology Co., Ltd., Dalian, Liaoning, China) and quantified via a Spectrophotometer (NanoDrop2000, Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription was carried out to synthesis cDNA following the instructions of the HiScript II Q RT SuperMix (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China).

    Article Title: PEX11β-mediated improvement of mitochondrial dysfunction restores behavioral defect and cellular viability in neuropathological conditions
    Article Snippet: .. Total RNA was isolated from HTT and AD cell lines using the Trizol method (RNAiso Plus-Takara) at the indicated hours (12, 24, 48hrs), while the quantity and quality of RNA were determined using the NanoDrop (Thermo Scientific). .. Using the PrimeScript 1st strand cDNA synthesis kit (Takara), 1μg of total RNA was reverse transcribed to prepare the respective cDNA.

    Article Title: Analyzing Fusarium graminearum strains for sensitivity to Tebuconazole fungicide and host responses by biochemical and molecular screening
    Article Snippet: Fusarium graminearum is the chief causal agent of Fusarium head blight (FHB) disease in 25 cereal crops.. The study aimed at identifying Tebuconazoleresistant and sensitive F. 26 graminearum strains and host responses using Arabidopsis thaliana-F. graminearum 27 interaction through biochemical and gene expression studies.. Thirty-two fungal isolates 28 isolated from FHB-infected wheat spikes were confirmed as F. graminearum members by 29 molecular and phylogenetic analyses.

    Spectrophotometry:

    Article Title: Tributyrin enhances growth and intestinal health in green mud crab ( Scylla paramamosain ) through butyrate-driven metabolic regulation
    Article Snippet: .. Briefly, total RNA (approximately 0.1 g sample) was isolated from the intestine and hepatopancreas of crabs (pooled from two crabs per replicate, n = 6 per treatment) using the Trizol method (RNAiso Plus 9108, Takara Biomedical Technology Co., Ltd., Dalian, Liaoning, China) and quantified via a Spectrophotometer (NanoDrop2000, Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription was carried out to synthesis cDNA following the instructions of the HiScript II Q RT SuperMix (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China).

    Article Title: Tributyrin enhances growth and intestinal health in green mud crab ( Scylla paramamosain ) through butyrate-driven metabolic regulation.
    Article Snippet: .. Briefly, total RNA (approximately 0.1 g sample) was isolated from the intestine and hepatopancreas of crabs (pooled from two crabs per replicate, n = 6 per treatment) using the Trizol method (RNAiso Plus 9108, Takara Biomedical Technology Co., Ltd., Dalian, Liaoning, China) and quantified via a Spectrophotometer (NanoDrop2000, Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription was carried out to synthesis cDNA following the instructions of the HiScript II Q RT SuperMix (Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China).

    Control:

    Article Title: Novel small molecule derivatives improve survivability in the cellular model of Huntington's disease via improving mitochondrial fusion
    Article Snippet: .. Total RNA was isolated from control and HD cells using the Trizol method (RNAiso Plus-Takara), while the quantity and quality of RNA were determined using NanoDrop (Thermo Scientific). cDNA was prepared using iScriptTM cDNA Synthesis (Bio-Rad) . .. RT-qPCR was carried out in a CFX96-real-time PCR machine (Bio-Rad) using SYBR green master mix (TB Green, Takara) with specific primers for DNM1L (DRP1), MFN1, MFF, FIS1, and PGC1α-Exon1a 25 (primer details in Table S3 under SI).

    cDNA Synthesis:

    Article Title: Novel small molecule derivatives improve survivability in the cellular model of Huntington's disease via improving mitochondrial fusion
    Article Snippet: .. Total RNA was isolated from control and HD cells using the Trizol method (RNAiso Plus-Takara), while the quantity and quality of RNA were determined using NanoDrop (Thermo Scientific). cDNA was prepared using iScriptTM cDNA Synthesis (Bio-Rad) . .. RT-qPCR was carried out in a CFX96-real-time PCR machine (Bio-Rad) using SYBR green master mix (TB Green, Takara) with specific primers for DNM1L (DRP1), MFN1, MFF, FIS1, and PGC1α-Exon1a 25 (primer details in Table S3 under SI).



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